Research in our laboratory focuses on fluid mechanics and transport phenomena in biological and geological porous media. We use theoretical, computational, and experimental approaches to measure and model transport rates and determine their influence in physiology, pathophysiology, resource extraction, and contaminant transport. We also use these transport fundamental to develop new diagnostic and drug delivery technologies with applications for cardiovascular diseases. A few of the current projects are described below.
Systems biology of genetic bleeding disorders. Hemophilia and von Willebrand disease are the most common genetic bleeding disorders. They both lead to reduced levels or dysfunction in key proteins that regulate blood clot formation. Despite detailed knowledge of molecular mechanisms, it is still difficult to predict bleeding risk in individuals in these disorders. In this project we take a systems biology approach to determine which biochemical and biophysical mechanisms modify the primary deficiency. In collaboration with Aaron Fogelson at University of Utah, Karin Leiderman at University of California, Merced, and Jorge Di Paola at University of Colorado, Denver. Funded by the National Institutes of Health.
Mass transfer regulation of blood clot formation. Coagulation is the biochemical pathway that works in concert with platelets to form a stable blood clot through the formation of the biopolymer fibrin. In this project, we are examining mass transfer limitations of various reactions and groups of reactions in coagulation to better understand the pathophysiology of bleeding and thrombotic disorders, as well their treatment with pro- and anticoagulation drugs. Funded by the American Heart Association and National Science Foundation.
Colloid-mediated radionuclide transport in heterogeneous porous media. Radionuclides adsorbed to natural and synthetic colloids, for example clays, can travel through soils at rates faster than water. However, the relative role of various transport, adsorption, and reaction processes is unknown. To address this gap in the knowledge base, the approach in this project is to use computational models, microscale soil analogs, and column experiments to determine the rate-limiting mechanisms that dictate colloid transport in soils with physical and chemical heterogeneities. In collaboration with Ning Wu and Xiaolong Yin at CSM and Wooyong Um and Jaehun Chun at Pacific Northwest National Laboratory. Funded by the Department of Energy.
Magnetically powered colloid assemblies to lyse blood clots. The dissolution of occlusive blood clots in small vessels is challenging because they are not accessible to catheter-based thrombectomy devices and diffusion of fibrinolytic agents to and through the clot can take hours to days. In this project we are using a novel propulsion mechanism discovered in our laboratory that relies on wheel-like motion of colloidal assemblies in rotating magnetic fields to target and ablate occlusive clots in the cardiovascular system. In collaboration with David Marr at CSM and Paco Herson at University of Colorado, Denver. Funded by the National Institutes of Health.
Visit our lab web page for a more information on current projects in the lab.
O. Tasci, P.S. Herson, K.B. Neeves, D.W.M Marr. Surface-enabled propulsion and control of colloidal microwheels. Nature Communications, 7 (2016): 10225.
A.L. Fogelson, K.B. Neeves. Fluid mechanics of blood clot formation. Annual Review of Fluid Mechanics, 47 (2015): 377-403.
A.R. Wufsus, K. Rana, A. Brown, J.R. Dorgan, M.W. Liberatore, K.B. Neeves. Elastic behavior and platelet retraction in low and high density fibrin gels. Biophysical Journal, 108 (2015): 173-183.
J.L. Sylman, D.T. Artzer, K. Rana, K.B. Neeves. A vascular injury model using focal heat-induced activation of endothelial cells. Integrative Biology, 15 (2015): 801-814.
A.A. Onasoga-Jarvis, T.J. Puls, S.K. O’Brien, L Kuang, H.J. Liang, K.B. Neeves. Thrombin generation and fibrin formation under flow on biomimetic tissue factor rich surfaces. Journal of Thrombosis and Haemostasis,12 (2014): 372-382.