Keith Neeves

Associate Professor, Chemical and Biological Engineering

Keith NeevesResearch in our laboratory focuses on fluid mechanics and transport phenomena in biological and geological porous media. We use theoretical, computational, and experimental approaches to measure and model transport rates and determine their influence in physiology, pathophysiology, resource extraction, and contaminant transport. We also use these transport fundamental to develop new diagnostic and drug delivery technologies with applications for cardiovascular diseases. A few of the current projects are described below.

Systems biology of genetic bleeding disorders. Hemophilia and von Willebrand disease are the most common genetic bleeding disorders. They both lead to reduced levels or dysfunction in key proteins that regulate blood clot formation. Despite detailed knowledge of molecular mechanisms, it is still difficult to predict bleeding risk in individuals in these disorders. In this project we take a systems biology approach to determine which biochemical and biophysical mechanisms modify the primary deficiency. In collaboration with Aaron Fogelson at University of Utah, Karin Leiderman at University of California, Merced, and Jorge Di Paola at University of Colorado, Denver. Funded by the National Institutes of Health.

Colloid-mediated radionuclide transport in heterogeneous porous media. Radionuclides adsorbed to natural and synthetic colloids, for example clays, can travel through soils at rates faster than water. However, the relative role of various transport, adsorption, and reaction processes is unknown. To address this gap in the knowledge base, the approach in this project is to use computational models, microscale soil analogs, and column experiments to determine the rate-limiting mechanisms that dictate colloid transport in soils with physical and chemical heterogeneities. In collaboration with Ning Wu and Xiaolong Yin at CSM and Wooyong Um and Jaehun Chun at Pacific Northwest National Laboratory. Funded by the Department of Energy.

Magnetically powered colloid assemblies to lyse blood clots. The dissolution of occlusive blood clots in small vessels is challenging because they are not accessible to catheter-based thrombectomy devices and diffusion of fibrinolytic agents to and through the clot can take hours to days. In this project we are using a novel propulsion mechanism discovered in our laboratory that relies on wheel-like motion of colloidal assemblies in rotating magnetic fields to target and ablate occlusive clots in the cardiovascular system. In collaboration with David Marr at CSM and Paco Herson at University of Colorado, Denver. Funded by the National Institutes of Health.

Visit our lab web page for a more information on current projects in the lab.

Contact

429 Alderson Hall
1613 Illinois Street
Golden, CO 80401
Office: (303) 273-3191
FAX: (303) 273-3730
kneeves@mines.edu

Research Group

  • Dr. Rogier Schoeman: PhD University of Twente
  • Yang Guo: Carnegie Mellon
  • Matt Sorrels: West Virginia
  • Dante Disharoon: Colorado School of Mines

Neeves Research Group Website

Education

  • BS – University of Colorado, Boulder
  • PhD – Cornell University
  • Post-Doctoral Study – University of Pennsylvania

Selected Publications

  • M. Lehmann, R.M. Schoeman, P.J. Krohl, A.M. Wallbank, J.R. Samaniuk, M. Jandrot-Perrus, K.B. Neeves. Platelets drive thrombus growth in a hematocrit and glycoprotein VI dependent manner in an in vitro model of venous thrombosis.Arteriosclerosis, Thrombosis, and Vascular Biology, 38 (2018): 1052-1062. DOI: 10.1161/ATVBAHA.118.310731
  • T.O. Tasci, D. Disharoon, R.M. Schoeman, K. Rana, P.S. Herson, D.W.M. Marr, K.B. Neeves. Enhanced fibrinolysis with magnetically powered colloidal microwheels. Small, 13 (2017): 1700954. DOI: 10.1002/smll.201700954
  • R.M. Schoeman, K. Rana, N. Danes, M. Lehmann, J.A. Di Paola, A.L. Fogelson, K. Leiderman, K.B. Neeves. A microfluidic model of hemostasis sensitive to platelet function and coagulation. Cellular and Molecular Bioengineering, 10 (2017): 3-15. DOI:10.1007/s12195-016-0469-0.
  • J.L. Sylman, D.T. Artzer, K. Rana, K.B. Neeves. A vascular injury model using focal heat-induced activation of endothelial cells. Integrative Biology, 15 (2015): 801-814.
  • A.R. Wufsus, K. Rana, A. Brown, J.R. Dorgan, M.W. Liberatore, K.B. Neeves. Elastic behavior and platelet retraction in low and high density fibrin gels. Biophysical Journal, 108 (2015): 173-183.

Google Scholar Citations Page

Honors and Awards

  • 2018 Cellular and Molecular Bioengineering Editors’ Choice Award
  • 2014 NSF CAREER Award
  • 2014 Young Innovator Award, Cellular and Molecular Bioengineering, Biomedical Engineering Society
  • 2013 Educator of the Year, Colorado BioScience Association
  • 2012 Early Career Investigator, Bayer Hemophilia Awards Program
  • 2010 Early Career Investigator of the Boettcher Foundation’s Webb-Waring Biomedical Research Program
Complete List of Publications
  1. Y. Tian, X. Yu, J. Li, X. Yin, K.B. Neeves, Y. Wu. Scaling law for slip flow of gases in nanoporous media from nanofluidics, rock, and pore-scale simulations. Fuel, in press.
  2. K.G. Link, M.T. Stobb, J. Di Paola, K.B. Neeves, A.L. Fogelson, S.S. Sindi, K. Leiderman. A local and global sensitivity analysis of a mathematical model of coagulation and platelet deposition under flow. PLoS One, 13 (2018): e0200917. DOI: 10.1371/journal.pone.0200917.
  3. K. Rod, W. Um, J. Chun, N. Wu, X. Yin, K.B. Neeves. Effect of chemical and physical heterogeneities on colloid-facilitated cesium transport. Journal of Contaminant Hydrology, 213 (2018):22-27. DOI: 10.1016/j.jconhyd.2018.03.012.
  4. M. Lehmann, R.M. Schoeman, P.J. Krohl, A.M. Wallbank, J.R. Samaniuk, M. Jandrot-Perrus, K.B. Neeves. Platelets drive thrombus growth in a hematocrit and glycoprotein VI dependent manner in an in vitro model of venous thrombosis. Arteriosclerosis, Thrombosis, and Vascular Biology, 38 (2018): 1052-1062. DOI: 10.1161/ATVBAHA.118.310731.
  5. B.R. Branchford, T.J. Stalker, L. Law, G. Acevedo, S. Sather, C. Brzezinski, K.M. Wilson, K. Minson, A.B. Lee-Sherick, P. Davizon-Castillo, C. Ng, W. Zhang, K.B. Neeves, S.R. Lentz, X. Wang, S.V. Frye, H.S. Earp III, D. DeRyckere, L.F. Brass, D.K. Graham, J.A. Di Paola. The small molecule MERTK inhibitor UNC2025 decreases platelet activation and prevents thrombosis. Journal of Thrombosis and Haemostasis, 16 (2018): 352-363. DOI: 10.1111/jth.13875.
  6. M. Lehmann, K. Ashworth, M. Manco-Johnson, J.A. Di Paola, K.B. Neeves, C.J. Ng. Evaluation of a microfluidic flow assay to screen for von Willebrand disease and low von Willebrand factor levels. Journal of Thrombosis and Haemostasis, 16 (2018): 104-115. DOI: 10.1111/jth.13881.
  7. T.O. Tasci, D. Disharoon, R.M. Schoeman, K. Rana, P.S. Herson, D.W.M. Marr, K.B. Neeves. Enhanced fibrinolysis with magnetically powered colloidal microwheels. Small, 13 (2017): 1700954. DOI: 10.1002/smll.201700954
  8. S. Cooper, S. Lloyd, A. Koch, X. Lin, K. Dobbs, T. Theisen, M. Zuberbuehler, T. Tenpas, K. Bernhardt, M. Gyorfi, S. Hying, S. Mortimer, C. Lamont, M. Lehmann, K. Neeves. Temperature effects on the activity, shape, and storage of platelets from 13-lined ground squirrels. Journal of Comparative Physiology B, 187 (2017): 815-825.
  9. R. M. Schoeman, M. Lehmann, K.B. Neeves. Flow chamber and microfluidic approaches for measuring thrombus formation in genetic bleeding disorders. Platelets, 28 (2017): 463-471.
  10. T. Yang, T.O. Tasci, K.B. Neeves, N. Wu, D.W.M. Marr. Magnetic microlassos for reversible cargo capture, transport, and release. Langmuir, 33 (2017): 5932-5937. Cover Article.
  11. B.L. Walton, M. Lehmann, T. Skorczewski, L.A. Holle, J.D. Beckman, J.A. Cribb, M.J. Mooberry, A.R. Wufsus, B.C. Cooley, J.W. Homeister, R. Pawlinski, M.R. Falvo, A.L. Falvo, N.S. Key, A.L. Fogelson, K.B. Neeves, A.S. Wolberg. Elevated hematocrit enhances platelet accumulation following vascular injury. Blood, 129 (2017): 2537-2546.
  12. M.U. Naik, P. Patel, R. Derstine, R. Turaga, X. Chen, K. Golla, K.B. Neeves, H. Ichijo, U.P. Naik. Apoptosis signal-regulating kinase 1 regulates platelet granule secretion, thromboxane A2generation, and thrombus formation in mice. Blood, 129 (2017): 1197-1209.
  13. R.M. Schoeman, K. Rana, N. Danes, M. Lehmann, J.A. Di Paola, A.L. Fogelson, K. Leiderman, K.B. Neeves. A microfluidic model of hemostasis sensitive to platelet function and coagulation. Cellular and Molecular Bioengineering, 10 (2017): 3-15. DOI:10.1007/s12195-016-0469-0.
  14. L. Wang, X. Yin, K.B. Neeves, E. Ozkan. Effect of pore-size distribution on phase transition of hydrocarbon mixtures in nanoporous media. SPE Journal, 21 (2016): 1981-1995.
  15. F.J. Maier, T. Lachner, A. Vilfan, T.O. Tasci, K.B. Neeves, D.W.M. Marr, and T.M. Fischer. Non reciprocal skewed rolling of a colloidal wheel due to induced chirality. Soft Matter, 12 (2016): 9314-9320.
  16. Y. Guo, J. Huang, F. Xiao, X. Yin, J. Chun, W. Um, K.B. Neeves, N. Wu. Bead-based microfluidic sediment analogs: Fabrication and colloid transport. Langmuir, 32 (2016): 9342-9350. DOI:10.1021/acs.langmuir.6b02184.
  17. K. Rana, K.B. Neeves. Blood flow and mass transfer regulation of coagulation. Blood Reviews, 30 (2016): 357-368. DOI: 10.1016/j.blre.2016.04.004
  18. K.B. Roth, K.B. Neeves, J. Squier, D.W.M. Marr. High-throughput linear optical stretcher for mechanical characterization of blood cells. Cytometry: Part A, 89 (2016): 391-397. DOI: 10.1002/ctyo.a.22794. Cover Article.
  19. O.J.T. McCarty, D. Ku, M. Sugimoto, M.R. King, J. Cosemans, K.B. Neeves. Dimensional analysis and scaling relevant to flow models of thrombus formation. Journal of Thrombosis and Haemostasis, 14 (2016): 619-622.
  20. T.O. Tasci, P.S. Herson, K.B. Neeves, D.W.M Marr. Surface-enabled propulsion and control of colloidal microwheels. Nature Communication, 7 (2016): 10225. DOI:10.1038/ncomms10225.
  21. S. Zhu, B.A. Herbig, R. Li, T.V. Colace, R.W. Muthard, K.B. Neeves, S.L. Diamond. In microfluidico: Recreating in vivohemodynamics using miniaturized devices. Biorheology, 52 (2015): 303-318. DOI; 10.3233/BIR-15065.
  22. M. Lehmann, A.M. Wallbank, K.A. Dennis, A.R. Wufsus, K.M. Davis, K. Rana, K.B. Neeves. On-chip recalcification of citrated whole blood using a microfluidic herringbone mixer.Biomicrofluidics, 6 (2015): 064106.
  23. K. He, L. Xu, Y. Gao, X. Yin, K.B. Neeves. Evaluation of surfactant performance in fracturing fluids for enhanced well productivity in the unconventional reservoirs using rock-on-a-chip approach. Journal of Petroleum Science and Engineering, 136 (2015): 531-541.
  24. K.B. Neeves. Physiochemical artifacts in FeCl3thrombosis models. Blood, 126 (2015): 700-701.
  25. J.L. Sylman, D.T. Artzer, K. Rana, K.B. Neeves. A vascular injury model using focal heat-induced activation of endothelial cells. Integrative Biology, 15 (2015): 801-814.
  26. B.R. Branchford, C.J. Ng, K.B. Neeves, J.A. Di Paola. Microfluidic technology as an emerging clinical tool to evaluate thrombosis and hemostasis. Thrombosis Research, 136 (2015): 13-19.
  27. K.B. Roth, K.B. Neeves, J. Squier, D.W.M. Marr. Imaging of a linear diode bar for an optical cell stretcher. Biomedical Optics Express, 6 (2015): 807-814.
  28. K.B. Neeves. Down in a hole: A new laser ablation model of hemostasis. Journal of Thrombosis and Haemostasis, 13 (2015): 414-416.
  29. A.R. Wufsus, K. Rana, A. Brown, J.R. Dorgan, M.W. Liberatore, K.B. Neeves. Elastic behavior and platelet retraction in low and high density fibrin gels. Biophysical Journal, 108 (2015): 173-183.
  30. A.L. Fogelson, K.B. Neeves. Fluid mechanics of blood clot formation. Annual Review of Fluid Mechanics, 47 (2015): 377-403.
  31. Q. Wu, B. Bai, Y. Ma, J.T. Ok, K.B. Neeves, X. Yin. Optic imaging of two-phase flow behavior in one-dimensional nano-scale channels. SPE Journal (2014), 19: 793-802.
  32. K. Rana, B.J. Timmer, K.B. Neeves. A combined microfluidic-microstencil method for patterning molecules and cells. Biomicrofluidics, 8 (2014): 056502.
  33. J.N. Thon, L. Mazutis, S Wu, J.L. Sylman, A. Ehrlicher, K.R. Machlus, Q. Feng, S. Lu, R. Lanza, K.B. Neeves, D.A. Weitz, J.E. Italiano Jr. Platelet bioreactor-on-a-chip. Blood, 124 (2014): 1857-1867.
  34. W. Xu, J.T. Ok, F. Xiao, K.B. Neeves, X. Yin. The effect of pore geometry and interfacial tension on water-oil displacement efficiency in oil-wet microfluidic porous media analogs. Physics of Fluids, 26 (2014): 093102.
  35. J.L. Sylman, S.M. Lantvit, M.M. Reynolds, K.B. Neeves. The relative role of soluble guanylyl cylase dependent and independent pathways in nitric oxide inhibition of platelet aggregation under flow. Cellular and Molecular Bioengineering, 7 (2014): 421-431. Selected for Young Innovators Special Issue.
  36. S. Ahmad, A. Ahmad, K.B. Neeves, J.E. Loader, T. Hendry-Hofer, C.W. White, L. Veress. In vitro cell culture model for toxic inhaled chemical testing. Journal of Visualized Experiments, 87 (2014): e51539. doi:10.3791/51539.
  37. A.A. Onasoga-Jarvis, T.J. Puls, S.K. O’Brien, L Kuang, H.J. Liang, K.B. Neeves.Thrombin generation and fibrin formation under flow on biomimetic tissue factor rich surfaces. Journal of Thrombosis and Haemostasis,12 (2014): 372-382.
  38. K.B. Neeves, O.J.T. McCarty, A.J. Reininger, M. Sugimoto, M.R. King. Flow-dependent thrombin and fibrin generation: Opportunities for standardization. Journal of Thrombosis and Haemostasis, 12 (2014): 418-420.
  39. A.A. Onasoga-Jarvis, K. Leiderman, A.L. Fogelson, M. Wang, M.J. Manco-Johnson, J.A. Di Paola, K.B. Neeves. The effect of factor VIII deficiencies and replacement and bypass therapies on thrombus formation under venous flow conditions in microfluidic and computational models. PLoS One, 8 (2013): e78732.
  40. J.L. Sylman, S.M. Lantvit, M.C. VeDepo, M.M. Reynolds, K.B. Neeves. Transport limitations of nitric oxide inhibition of platelet aggregation under flow. Annals of Biomedical Engineering, 41 (2013): 2193-2205.
  41. J.L. Sylman, K.B. Neeves. An inquiry-based investigation of controlled release drug delivery from hydrogels: An experiment for high school chemistry and biology. Journal of Chemical Education, 90 (2013): 918-921.
  42. K.B. Neeves, A.A. Onasoga, A.R. Wufsus. The use of microfluidics in hemostasis: Clinical diagnostics and biomimetic models of vascular injury. Current Opinion in Hematology, 20 (2013):417-423.
  43. A.R. Wufsus, N.E. Macera, K.B. Neeves. The hydraulic permeability of blood clots as a function of fibrin and platelet density. Biophysical Journal, 104 (2013): 1812-1823. Featured in New and Notable editorial.
  44. K.B. Roth, C.D. Eggleton, K.B. Neeves, D.W.M. Marr. Measuring cell mechanics by optical alignment compression cytometry. Lab on a Chip, 13 (2013): 1571-1577.
  45. Q. Wu, J.T. Ok, Y. Sun, S.T. Retterer, K.B. Neeves, X. Yin, B. Bai, Y. Ma. Optic imaging of single and two-phase pressure driven flow in nano-scale channels. Lab on a Chip, 13 (2013): 1165-1171.
  46. K.B. Neeves, A.A. Onasoga, R.R. Hansen, J.J. Lilly, D. Venckunaite, M.B. Sumner, A.T. Irish, G. Brodsky, M.J. Manco-Johnson, J.A. Di Paola. Sources of variability in platelet accumulation on type I fibrillar collagen in microfluidic flow assays. PLoS One, 8 (2013): e54680. doi:10.1371/journal.pone.0054680.
  47. R.R. Hansen, A.R. Wufsus, S.T. Barton, A.A. Onasoga, R.M. Johnson-Paben, K.B. Neeves. High content analysis of shear dependent platelet function in a microfluidic flow assay. Annals of Biomedical Engineering, 14 (2013): 250-262.
  48. S. Ahmad, A. Ahmad, R.C. Rancourt, K.B. Neeves, J.E. Loader, T. Hendry-Hofer, J. Di Paola, S.D. Reynolds, C.W. White. Tissue factor signals airway epithelial basal cell survival via coagulation and PAR1/2. American Journal of Respiratory Cell and Molecular Biology, 48 (2013): 94-104.
  49. K.B. Neeves. Collagen-induced thrombosis in large vessels using native prothrombotic substrates.Thrombosis Research, 131 (2013), 1-2.
  50. V.K. Gupta, K.B. Neeves, C.D. Eggleton. Effect of viscoelasticity on the analysis of single molecule force spectroscopy on live cells.Biophysical Journal, 103 (2012), 137-145.
  51. K.B. Neeves. Catch me if you can: Isolating circulating tumor cells from flowing blood. Clinical Chemistry, 58 (2012), 803-804.
  52. C.P. Foley, N. Nishimura, K.B. Neeves, C.B. Schaffer, W.L. Olbricht. Real-time imaging of perivascular transport of nanoparticles during convection enhanced delivery in the rat cortex. Annals of Biomedical Engineering, 40 (2012), 292-303.
  53. S. Ahmad, D. O. Raemy, J. E. Loader, J. M. Kailey, K. B. Neeves, C. W. White, A. Ahmad, P. Gehr, B. M. Rothen-Rutishauser. Interaction and localization of synthetic nanoparticles in healthy and cystic fibrosis airway epithelial cells: Effect of ozone exposure. Journal of Aerosol Medicine and Pulmonary Drug Delivery, 25(2012), 7-15.
  54. M. Wu, F. Xiao, R.M. Johnson-Paben, S.T. Retterer, X. Yin, K.B. Neeves. Single- and two-phase flow in microfluidic porous media analogs based on Voronoi tessellation.  Lab on a Chip, 12 (2012), 253-261. Cover Article.
  55. R.R. Hansen, A.A. Tipnis, T.C. White-Adams, J.A. Di Paola, K.B. Neeves. Characterization of collagen thin films for von Willebrand factor binding and platelet adhesion.  Langmuir, 27 (2011), 13648-13658.
  56. K.M. Wannemacher, L. Zhu, H. Jiang, K.P. Fong, T.J. Stalker, D. Lee, A.N. Tran, K.B. Neeves, S. Maloney, A. Kumanogoh, H. Kikutani, D.A. Hammer, S.L. Diamond, L.F. Brass. Diminished contact-dependent reinforcement of Syk activation underlies impaired thrombus growth in mice lacking Semaphorin 4D.  Blood, 116 (2010), 5707-5715.
  57. W.L. Olbricht, K.B. Neeves, C.P. Foley.Microfluidic probes in the treatment of brain-related diseases.  Drug News and Perspectives, 23 (2010), 491-497.
  58. K.B. Neeves, D.A.R. Illing, S.L. Diamond. Thrombin flux and wall shear rate regulate fibrin fiber deposition state during polymerization under flow.  Biophysical Journal, 98 (2010), 1344-1352.
  59. A.A. Schmaier, Z. Zou, A. Kazlauskas, L. Emert-Sedlak, K.P. Fong, K.B. Neeves, S.F. Maloney, S.L. Diamond, S.P. Kunapuli, J. Ware, L.F. Brass, T.E. Smithgall, K. Saksela, M.L. Kahn. Molecular priming of Lyn by GPVI enables an immune receptor to adopt a hemostatic role.  Proceedings of the National Academy of Sciences of the USA, 106 (2009), 21167-21172.
  60. L. Zhu, T.J. Stalker, K.P. Fong, H. Jiang, A. Tran, I. Crichton, E.K. Lee, K.B. Neeves, S.F. Maloney, H. Kikutani, A. Kumanogoh, E. Pure, S.L. Diamond, and L.F. Brass. Disruption of SEMA4D ameliorates platelet hypersensitivity in dyslipidemia and confers protection against the development of atherosclerosis.Arteriosclerosis, Thrombosis, and Vascular Biology, 29 (2009), 1039-1045.
  61. C.P. Foley, N. Nishimura, K.B. Neeves, C.B. Schaffer, and W.L. Olbricht.Flexible microfluidic devices supported by biodegradable insertion scaffolds for convection-enhanced neural drug delivery.  Biomedical Microdevices, 11 (2009), 915-924.
  62. K.B. Neeves, S.F. Maloney, K.P. Fong, A.A. Schmaier, M.L. Kahn, L.F. Brass, and S. L. Diamond. Microfluidic focal thrombosis model for measuring murine platelet deposition and stability: PAR4 signaling enhances shear-resistance of platelet aggregates. Journal of Thrombosis and Haemostasis, 6 (2008), 2193-2201.
  63. K.B. Neeves and S.L. Diamond. A membrane-based microfluidic device for controlling the flux of platelet agonists into flowing blood. Lab on a Chip, 8 (2008), 701-709.
  64. U.M. Okorie, W.S. Denney, M.S. Chatterjee, K.B. Neeves, and S.L. Diamond. Determination of surface tissue factor thresholds that trigger coagulation at venous and arterial shear rates: amplification of 100 fM circulating tissue factor requires flow. Blood, 111 (2008), 3507-3513.
  65. K.B. Neeves, A.J. Sawyer, C.P. Foley, W.M. Saltzman, and W.L. Olbricht. Dilation and degrada­tion of the brain extracellular matrix enhances penetration of infused polymer nanoparticles. Brain Research, 1180 (2007), 121-132.
  66. A.J.H. Spence, K.B. Neeves, D. Murphy, S. Sponberg, B. Land, R.R. Hoy, and M.S. Isaacson. Flex­ible multielectrodes can resolve multiple muscles in an insect appendage. Journal of Neuroscience Methods159 (2007), 116-124.
  67. K.B. Neeves, C.T. Lo, C.P. Foley, W.M. Saltzman, and W.L. Olbricht. Fabrication and character­ization of microfluidic probes for convection enhanced drug delivery. Journal of Controlled Release, 111 (2006), 252-262.
  68. S.T. Retterer, K.L. Smith, C.S. Bjornsson, K.B. Neeves, A.J.H. Spence, J.N. Turner, W. Shain, and M.S. Isaacson. Model neural prostheses with integrated microfluidics: A potential Intervention strategy for controlling reactive cell and tissue responses. IEEE Transactions on Biomedical Engineering, 51 (2004), 2063-2073.