Current molecular probes and sensor technologies present limitations in both the range of targets that can be measured and the ability to function in the complicated in vivo environment. My research program will help answer pressing biomedical questions by developing novel nanosensors that overcome these limitations and applying these new tools in situ. Traditional sensor designs, while being quite valuable for in vitro and laboratory analysis, often fail in vivo due to complications such as sensor invasiveness, sensitivity, selectivity, and limitations from the sensor readout mechanism. Polymeric nanosensors have emerged over the past several years as an excellent nanosensor design platform for continuous in vivo monitoring that overcomes the shortcomings of many traditional sensor designs.
Using this platform, I will focus my research on three distinct projects for which continuous monitoring can provide valuable information:
- Develop Enzyme Linked Nanosensors (ELiNS) to continuously monitor lactate levels during acute liver failure.
- Develop peptide targeted nanosensors for pH, pO2, pCO2, and lactate to monitor tumor metabolism during treatment.
- Develop fluorescence lifetime based nanosensors for improved cellular imaging in biofilms.
- BS – Northeastern University
- PhD – University of California, Santa Barbara
- Post-Doctoral Study – C.S. Draper Laboratory and Northeastern University
- Cash, KJ, Clark, HA, “Phosphorescent Nanosensors for in Vivo Tracking of Histamine Levels”, Analytical Chemistry, 2013, 85, 6312-6318.
- Cash, KJ, Clark, HA, “In Vivo Histamine Optical Nanosensors”, Sensors, 2012, 12, 11922-11932, PMCID.
- Cash, KJ, Clark, HA, “Nanosensors and Nanomaterials for Monitoring Glucose in Diabetes”, Trends in Molecular Medicine, 2010, 16, 584-593, PMCID.
- Cash, KJ, Ricci, F, Plaxco, KW, “A General Electrochemical Method for Label-Free Screening of Protein-Small Molecule Interactions”, Chem. Commun. 2009, 6222-6224, PMCID.
- Cash, KJ, Ricci, F, Plaxco, KW, “An Electrochemical Sensor for the Detection of Protein-Small Molecule Interactions Directly in Serum and Other Complex Matrices”, JACS, 2009, 131, 6955-6957, PMCID.
- Cash, KJ, Heeger, AJ, Plaxco, KW, Xiao, Y, “Optimization of a Reusable, DNA Pseudoknot-Based Electrochemical Sensor for Sequence-Specific DNA Detection in Blood Serum”, Analytical Chemistry, 2009, 81, 656-661, PMCID.